Saos-2 ("sarcoma osteogenic") is a cell line derived from the primary osteosarcoma of an 11-year-old Caucasian girl in 1973 by Fogh et al.[1] The cell line is commonly used in bone cancer research as a model for testing novel therapies.[2]

In 1987 Rodan et al. determined that Saos-2 cells "possess several osteoblastic features and could be useful as a permanent line of human osteoblast-like cells and as a source of bone-related molecules."[3]

Besides their worldwide availability, some of the advantages for using Saos-2 cell line are that they have well-documented characterization data, the possibility to obtain large amounts of cells in short time, and the fact that Saos-2 cells can be fully differentiated in a manner that the osteoblastic cells naturally do.[4] The latter point is described particularly as "the ability of Saos-2 cells to deposit a mineralization-competent extracellular matrix",[5] which makes these cells a valuable model for studying events associated with the late osteoblastic-osteocyte differentiation stage in human cells. [6] [7]

References

  1. ↑ Fogh J, Fogh JM, Orfeo T (1977). "One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice". J Natl Cancer Inst. 59 (1): 221–226. doi:10.1093/jnci/59.1.221. PMID 327080.
  2. ↑ "Saos-2". ATCC. Retrieved 11 June 2018.
  3. ↑ Rodan SB, Imai Y, Thiede MA, Wesolowski G, Thompson D, Bar-Shavit Z, Shull S, Mann K, Rodan GA (1987). "Characterization of a human osteosarcoma cell line (Saos-2) with osteoblastic properties". Cancer Res. 47 (18): 4961–6. PMID 3040234.
  4. ↑ Hausser HJ, Brenner RE (2005). "Phenotypic instability of Saos-2 cells in long-term culture". Biochem. Biophys. Res. Commun. 333 (1): 216–22. doi:10.1016/j.bbrc.2005.05.097. PMID 15939397.
  5. ↑ McQuillan DJ, Richardson MD, Bateman JF (1995). "Matrix deposition by a calcifying human osteogenic sarcoma cell line (SAOS- 2)". Bone. 16 (4): 415–26. doi:10.1016/8756-3282(95)90186-8. PMID 7605701.
  6. ↑ Gundle R, Beresford JN (1995). "The isolation and culture of cells from explants of human trabecular bone". Calcif. Tissue Int. 56 (Suppl 1): S8-10. doi:10.1007/BF03354640. PMID 7719993. S2CID 11507101.
  7. ↑ Prideaux M, Wijenayaka AR, Kumarasinghe DD, Ormsby RT, Evdokiou A, Findlay DM, Atkins GJ (2014). "Saos2 osteosarcoma cells as an in vitro model for studying the transition of human osteoblasts to osteocytes". Calcif. Tissue Int. 95 (2): 183–193. doi:10.1007/s00223-014-9879-y. PMID 24916279. S2CID 253747577.
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