Plastic Roux bottles.

A Roux culture bottle, or simply Roux bottle, is a type of laboratory glassware used in biology and related sciences to grow microorganisms or tissue cells.[1]: 5 [2]: 1886  It consists of a bottle of transparent glass[3] or plastic[4] with two closely spaced flat, rectangular, parallel faces and a short neck; of such a design that the bottle can be laid down sideways, on one of those two faces, even when unstoppered and partially filled with a culture medium. This goal is achieved by having the neck narrowed,[3] offset,[5] partially blocked,[6][7] or canted (tilted).[8][9] The item is also generically called cell culture bottle[4] or tissue culture (TC) bottle,[8] and flask may be used instead of "bottle".[3]

Pile of Roux bottles with culture medium.

A Roux bottle provides a large surface for the cells or microorganisms to grow, whether on the top of,[10] floating in,[11] or at the bottom of the medium.[12] The flat upper face then allows inspection of the culture and even illumination for photosynthetic organisms.[11] Organisms growing at the bottom of the medium can also be inspected from below, with an inverted microscope.[13]

The bottle is typically closed with a plug or cap that prevents contamination of the culture by spores from the outside, while still allowing exchange of gases generated or consumed by the organisms inside.[12]

The bottle's invention has been attributed to French physician and Pasteur's collaborator Pierre Roux (1853-1933).[14][15]

Variations

The "DeLong" type has a horizontal neck with a low barrier at the base.[7] There is a cylindrical version that is meant to be slowly tuned on rollers to keep the contents well mixed.[16]

See also

References

  1. ↑ John W. Eyre (1913): Bacteriological Techniques: A Laboratory Guide. Reprinted as Volume 39 of Historical Science series, Books on Demand, 2010; 556 pages. ISBN 9783867412568
  2. ↑ Christopher G. Morris, Christopher W. Morris (): Academic Press Dictionary of Science and Technology. 2432 pages. ISBN 9780122004001
  3. 1 2 3 Kisker Biotech GmbH (2020): "Roux flask 38 mm 1200 mL long neck". Online catalog item 202940. Accessed on 2020-02-21.
  4. 1 2 Carl Roth GmbH (2015): "Cell culture bottles Filter screw cap". Online catalog item CE48.1. Accessed on 2020-02-21.
  5. ↑ Sigma-Aldrich (2020): "Pyrex Roux culture flask, off-set neck" Online catalog entry SLW1350/04M. Accessed on 2020-02-21.
  6. ↑ Chemglass Co. (2020): "BOTTLES, ROUX, 1200ML". Online catalog item CLS-1195-1200. Accessed on 2020-02-21.
  7. 1 2 Bellco Glass (2020): "DeLong Roux Culture Bottle 1200mL Schott Blank". Online catalog item, SKU 5630-12000. Accessed on 2020-02-21.
  8. 1 2 Sarstedt Co. (2020): "TC Flask T175,Standard". Online catalog item 83.3912. Accessed on 2020-02-21.
  9. ↑ Orioner, Malaysia (2020): "Pigeons Type Cell Culture Bottle by Sichuan Shubo". Online catalog item 1481-145. Accessed on 2020-02-21.
  10. ↑ Marjory Stephenson and Margaret Dampier Whetham (1922): "Studies in the Fat Metabolism of the Timothy Grass Bacillus". Proceedings of the Royal Society of London, Series B - Biology, volume 93, pages 262-280. doi:10.1098/rspb.1922.0020
  11. 1 2 Blaine Carter and Michael Huesemann (2008): "Hydrogen production by the cyanobacterium Plectonema boryanum: Effects of initial nitrate concentration, light intensity, and inhibitionof photosystem II by DCMU". U.S. Department of Energy, Journal of Undergraduate Research, OSTI article 1052083, volume 8, pages 23-32.
  12. 1 2 Sophie A. Leliévre (2015): "Cell Culture Basics 11: Adding medium to a culture flask with attached cells". Lab technique training video produced by Purdue University, uploaded to YouTube.com on 2015-09-23. Accessed on 2020-02-21.
  13. ↑ Abnova (2011): "Cell Culture (Attached Cell)". Technical instruction video, uploaded to YouTube on 2011-01-13. Accessed on 2020-02-21.
  14. ↑ Erhard F. Kaleta (2006): "A Brief History, Modes of Spread and Impact of Fowl Plague Viruses". Asia-Pacific Biotech News, volume 10, issue 14, pages 717-725. doi:10.1142/S0219030306001236
  15. ↑ Erhard F. Kaleta and Catherine P. A. Rülke (2009): "The beginning and spread of Fowl Plague (H7 High Pathogenicity Avian Influenza) across Europe and Asia (1878-1955)". In David E. Swayne (ed), Avian Influenza. pages 145-189. ISBN 9780813818665 doi:10.1002/9780813818634.ch7
  16. ↑ Carl Roth GmbH (2015): "Cell culture bottles CELLMASTER Plastic roller bottles Filter screw cap". Online catalog item CE59.1. Accessed on 2020-02-21.
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