Pseudomonas denitrificans
Scientific classification Edit this classification
Domain: Bacteria
Phylum: Pseudomonadota
Class: Gammaproteobacteria
Order: Pseudomonadales
Family: Pseudomonadaceae
Genus: Pseudomonas
Species:
P. denitrificans
Binomial name
Pseudomonas denitrificans
Bergey, et al. 1961
Type strain
ATCC 19244

CCEB 525
IAM 12023
NCIMB 1656
NCTC 1656
NRRL B-1028

Pseudomonas denitrificans is a Gram-negative aerobic bacterium that performs denitrification. It was first isolated from garden soil in Vienna, Austria. It overproduces cobalamin (vitamin B12), which it uses for methionine synthesis[1] and it has been used for manufacture of the vitamin.[2] Scientists at Rhône-Poulenc Rorer took a genetically engineered strain of the bacteria, in which eight of the cob genes involved in the biosynthesis of the vitamin had been overexpressed, to establish the complete sequence of methylation and other steps in the cobalamin pathway.[3][4]

Based on 16S rRNA analysis, P. denitrificans has been placed in the P. pertucinogena group.[5]

References

  1. ↑ Lago BD, and Demain AL (1969). "Alternate requirement for vitamin B12 or methionine in mutants of Pseudomonas denitrificans, a vitamin B12-producing bacterium". J Bacteriol. 99 (1): 347–9. doi:10.1128/jb.99.1.347-349.1969. PMC 250011. PMID 5802615.
  2. ↑ Fang, H; Kang, J; Zhang, D (30 January 2017). "Microbial production of vitamin B12: a review and future perspectives". Microbial Cell Factories. 16 (1): 15. doi:10.1186/s12934-017-0631-y. PMC 5282855. PMID 28137297.
  3. ↑ Debussche, L.; Thibaut, D.; Cameron, B.; Crouzet, J.; Blanche, F. (1993). "Biosynthesis of the corrin macrocycle of coenzyme B12 in Pseudomonas denitrificans". Journal of Bacteriology. 175 (22): 7430–7440. doi:10.1128/jb.175.22.7430-7440.1993. PMC 206888. PMID 8226690.
  4. ↑ R. Caspi (2013-09-25). "Pathway: adenosylcobalamin biosynthesis II (aerobic)". MetaCyc Metabolic Pathway Database. Retrieved 2020-04-24.
  5. ↑ Anzai, Y.; Kim, H.; Park, J. Y.; Wakabayashi, H.; Oyaizu, H. (2000). "Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence". International Journal of Systematic and Evolutionary Microbiology. 50 (4): 1563–1589. doi:10.1099/00207713-50-4-1563. PMID 10939664.


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