| Intron-encoded endonuclease I-SceI | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | SCEI | ||||||
| PDB | 1R7M | ||||||
| UniProt | P03882 | ||||||
| |||||||
Intron-encoded endonuclease I-Sce I is a homing endonuclease. The enzyme is used in biotechnology as a meganuclease. It recognises an 18-base pair sequence TAGGGATAACAGGGTAAT and leaves a 4 base pair 3' hydroxyl overhang. It is a rare cutting endonuclease. Statistically an 18-bp sequence will occur once in every 6.9*1010 base pairs (a frequency of 1 in 418). This sequence does not normally occur in a human or mouse genome.[1]
Sources
I-SceI is coded by introns. It is present in the mitochondria of yeast Saccharomyces cerevisiae.
References
- ↑ New England Biolabs site Archived 2008-05-02 at the Wayback Machine
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