Sassafras is a genus of three species of trees native to North America and Asia.

Sassafras may also refer to:

Plants

• Atherospermataceae, or Southern Sassafras, a southern hemisphere family of trees
• Doryphora sassafras, an Australian tree from temperate rainforests
• Sassafras albidum, the sassafras tree of eastern North America, used in tea, root beer, and gumbo filé powder
• Cinnamomum oliveri, known as Black Sassafras or Oliver's Sassafras, an Australian rain forest laurel
• Street slang for Marijuana

Vessels

• USCGC Sassafras (WLB-401), United States Coast Guard buoy tender

Places

• Sassafras, New South Wales, a village in New South Wales, Australia
• Sassafras, Tasmania, a town in northern Tasmania, Australia
• Sassafras, Victoria, a city suburb of Melbourne, Victoria, Australia
• Sassafras, Indiana, an unincorporated community
• Sassafras, Maryland, a location in the United States
• Sassafras, West Virginia, an unincorporated community
• Sassafras River, a tributary of the Chesapeake Bay in Maryland and Delaware, United States
• Sassafras Mountain, the highest point in the state of South Carolina, United States

Music

• Sassafras (band), a rock band from South Wales formed in the 1970s
• Sassafrass!, a 2018 album by Tami Neilson
• "Sassafras", a song by The Devil Wears Prada from With Roots Above and Branches Below
• "Sassafras", a song by the Helio Sequence which appears on Com Plex
• "Sassafras Roots", a song by Green Day from Dookie
• "Sassafras", a song by Billy Edd Wheeler covered by Van Dyke Parks on Songs Cycled

Animals

• Sassafras (horse) (1967-1988), a racehorse