Pensacola is a city in the western part of the U.S. state of Florida.

Pensacola may also refer to:

Anthropology

• Pensacola people, a Native American people historically of western Florida
• Pensacola culture, an archaeological culture of the Mississippian period along the Gulf coast of the United States

Entertainment

• Pensacola: Wings of Gold, a television series
• "Pensacola", a 1995 song by Joan Osborne from Relish
• "Pensacola", a 1998 song by Soul Coughing from El Oso
• "Pensacola", a 2011 song by Manchester Orchestra from Simple Math
• "Pensacola", a 2013 song by Deerhunter from "Monomania"

Military

• Naval Air Station Pensacola, a major U.S. Navy base
• USS Pensacola (CA-24), a U.S. Navy heavy cruiser, the lead ship of her class of heavy cruiser
• Pensacola class cruiser, a class of U.S. Navy cruisers

Places

• Pensacola Bay, a bay in Florida
• Pensacola Beach, Florida, an unincorporated community and beach
• Historic Pensacola Village, an area of Pensacola, Florida
• Pensacola, Oklahoma

Other uses

• Pensacola (spider), a genus of jumping spiders
• Pensacola station (disambiguation), stations of the name
• Peniscola, Spain