Matherne is a French surname. Notable people with the surname include:
- Beverly Matherne (born 1946), American writer and poet
- Wayne Matherne (born 1949), retired Canadian football player
- Brian Matherne, a coach in a sex abuse case in the archdiocese of Gregory Michael Aymond
- Ethel Jeanne Matherne (1916–2002), wife of educator and businessman Cliff Ammons
- Londa Jean Matherne, wife of Harry Connick Sr.
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