Karlstein may refer to places in:

Germany

• Karlstein am Main, a municipality in Landkreis Aschaffenburg, Bavaria
• part of Bad Reichenhall, Bavaria
  • a rock with a ruin there
• Karlstein bei Hornberg, a rock in Hornberg, Baden-Württemberg
• part of Regenstauf, Landkreis Regensburg, Bavaria
  • Schloss Karlstein, the castle there
• Aussichtspunkt Karlstein, a site above Weinstadt, Baden-Württemberg

Austria

• Karlstein an der Thaya, a municipality in Lower Austria, Austria
  • Schloss Karlstein, the castle there
  • Internierungslager Karlstein an der Thaya, a former internment camp there

Czech Republic

• Karlštejn Castle, a 14th-century castle in Central Bohemia
  • Karlštejn (Beroun District), městys named after the castle
  • Karlštejn, nature reserve nearby the castle
• Hunting castle Karlštejn nearby village Svratouch in Pardubice Region (Chrudim District)
  • Karlštejn, administrative part of village Svratouch, named after the hunting castle
  • Karlštejn, hill in Žďárské vrchy where the hunting castle is situated