James Valentine may refer to:
- James Valentine (journalist) (fl. late 20th century), Australian; also musician
- James Valentine (musician) (born 1978), guitarist of American band Maroon 5
- James Valentine (photographer) (1815–1879), Scottish photographer
- Jim Valentine (1866–1904), English rugby union footballer
- James Valentine (RFC officer) (1887–1917), early English aviator and Royal Flying Corps pilot
- James W. Valentine (1926–2023), American evolutionary biologist
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