Isono (written: 磯野) is a Japanese surname. Notable people with the surname include:
- Isono Kazumasa (磯野 員昌, 1534–1583), Japanese samurai
- Kiriko Isono (磯野 貴理子, born 1964), Japanese comedian, entertainer and actress
- Nobuhisa Isono (磯野 修久, born 1974), Japanese footballer
- Isono Tamba-no-kami (磯野 丹波), Japanese samurai
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