E320 may refer to:
- Butylated hydroxyanisole (E number 320), a synthetic, waxy petrochemical
- British Rail Class 374 (branded as E320), A European high speed train in use on the Eurostar network
- Mercedes-Benz E-Class, a range of executive cars
- ThinkPad E series (Gen 2, model E320), a notebook computer released in 2010
- FS Class E.320, a class of Italian third rail electric locomotives, built in the 1910s
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