The Berlese funnel is used to extract organisms from soil.

A Berlese funnel, also known as Tullgren funnel, Berlese trap, or Berlese-Tullgren funnel, is an apparatus used to extract living organisms, particularly arthropods, from samples of soil. The Tullgren funnel works by creating a desiccation gradient over the sample such that mobile organisms will move away from the dry environment and fall into a collecting vessel, where they perish and are preserved for examination. The illustration shows how it works: a funnel (E) contains the soil or litter (D), and a heat source (F) such as an electric lamp (G) heats the litter. Animals escaping from the desiccation of the litter descend through a filter (C) into a preservative liquid (A) in a receptacle (B). This illustration is merely a schematic, since usually the soil sample will not be crumbled and poured into the funnel (this would inevitably lead to a high amount of soil particles in the preservation fluid requiring laborious work to sort out the soil organisms). In fact, the soil sample is placed on a mesh sieve that will allow the soil animals to pass but should retain most of the soil particles.

This type of extraction is commonly referred to as Berlese funnel or Tullgren funnel. Antonio Berlese described this method of dynamic sampling in 1905 with a hot water jacket as heat source.[1] In 1918 Albert Tullgren described a modification, where the heating came from above by an electric bulb and the heat gradient was increased by an iron sheet drum around the soil sample.[2] Today's extraction funnels of this type usually combine elements from both publications and thus should be referred to as Berlese-Tullgren funnel.[3]

A variation of the Berlese funnel uses naphthalene flakes or similar aromatic mothballs in place of the heat source to drive organisms downward.[4] This method finds application in situations without electrical power, where the organisms are repulsed by volatile preservatives in collection container, or they cannot migrate downward quickly enough to avoid desiccation.

See also

References

  1. ↑ Berlese, Antonio (1905). Apparecchio per raccogliere presto ed in gran numero piccoli Artropodi [Apparatus for gathering early and in large numbers small arthropods] (in Italian). pp. 85–90. OCLC 79048180.
  2. ↑ Tullgren, A (26 August 2009). "Ein sehr einfacher Ausleseapparat für terricole Tierformen" [A very simple readout device for terricole animal forms]. Zeitschrift für Angewandte Entomologie (in German). 4 (1): 149–150. doi:10.1111/j.1439-0418.1918.tb00820.x.
  3. ↑ Southwood, T.R.E. (1994). Ecological methods. Chapman & Hall. ISBN 978-0-412-30710-2.
  4. ↑ Brown, Richard D. (15 March 1973). "Funnel for Extraction of Leaf Litter Organisms". Annals of the Entomological Society of America. 66 (2): 485–6. doi:10.1093/aesa/66.2.485.

Further reading

  • Smith, L. B. (31 May 2012). "Efficiency of Berlese-Tullgren funnels for removal of the rusty grain beetle, Cryptolestes ferrugineus, from wheat samples". The Canadian Entomologist. 109 (4): 503–9. doi:10.4039/Ent109503-4.
  • Dondale, C. D.; Nicholls, C. F.; Redner, J. H.; Semple, R. B.; Turnbull, A. L. (31 May 2012). "An improved Berlese-Tullgren funnel and a flotation separator for extracting grassland arthropods". The Canadian Entomologist. 103 (11): 1549–52. doi:10.4039/Ent1031549-11.
  • Crossley, D.A.; Blair, John M. (February 1991). "A high-efficiency, 'low-technology' Tullgren-type extractor for soil microarthropods". Agriculture, Ecosystems & Environment. 34 (1–4): 187–92. doi:10.1016/0167-8809(91)90104-6.
  • Hubert, Jan; Nesvorna, Marta; Stejskal, Vaclav (October 2009). "The efficacy of sieving, filth flotation and Tullgren heat extraction for detecting various developmental stages of Tribolium castaneum and Ephestia kuehniella in samples of wheat grain, flour and semolina". Journal of Stored Products Research. 45 (4): 279–88. doi:10.1016/j.jspr.2009.05.003.
  • Wheeler, Quentin D.; McHugh, Joseph V. (1987). "A Portable and Convertible 'Moczarski/Tullgren' Extractor for Fungus and Litter Coleoptera". The Coleopterists Bulletin. 41 (1): 9–12. JSTOR 4008312.
  • Harris, Marvin (15 October 1971). "Sampling Pear Foliage for Nymphs of the Pear Psylla, Using the Berlese-Tullgren Funnel". Journal of Economic Entomology. 64 (5): 1317–8. doi:10.1093/jee/64.5.1317.
  • Besuchet, Claude; Burckhardt, Daniel H.; Löbl, Ivan (1987). "The 'Winkler/Moczarski' Eclector as an Efficient Extractor for Fungus and Litter Coleoptera". The Coleopterists Bulletin. 41 (4): 392–394. JSTOR 4008527.
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